Fig. 4From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli Extracellular medium SDS-PAGE analysis of the WT and variants. Lanes: M, protein molecular mass markers; p-M1, pelB-M1; p-M3, pelB-M3; p-M5, pelB-M5; p-WT, pelB-WT; M1; M3; M5; WT. The values are listed as molecular sizes in kilodaltonsBack to article page