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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Fig. 3

SDS-PAGE analysis of the WT and variants. Lanes: M 1 and M 2 , protein molecular mass markers; M1; M3; M5; WT. The values between the panels are listed in molecular sizes in kilodaltons. a Anionic chromatography purification on HiTrap Capto Q column (GE Healthcare, Sweden). b His-tag purification on complete His-Tag Purification Column (GE Healthcare, Sweden)

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