Fig. 3From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli SDS-PAGE analysis of the WT and variants. Lanes: M 1 and M 2 , protein molecular mass markers; M1; M3; M5; WT. The values between the panels are listed in molecular sizes in kilodaltons. a Anionic chromatography purification on HiTrap Capto Q column (GE Healthcare, Sweden). b His-tag purification on complete His-Tag Purification Column (GE Healthcare, Sweden)Back to article page