Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

Table 4 Strains and plasmids

Strain/plasmid	Characteristics	References
Strains
L. lactis CECT8666 (formerly GE2-24)	Isolated from an artisanal cheese, containing AGDI cluster	[31]
L. lactis NZ9000	Expression host for NICE system, lacking AGDI cluster	[29]
Plasmids
pNZ8048	Lactococcal plasmid containing the nisA promoter and the Cm^r	[29]
pNZErmC	pNZ8048 derivative containing the His-tag and the Cm^r	[6]
pUC57-pep	pUC57 derivative containing the pep gene from M. Xanthus and the Ap^r	[36]
pAGDI	pNZ8048 derivative bearing the P_aguR-aguR-P_aguB-gfp fusion and the Cm^r	[34]
pAG2	pNZ8048 derivative bearing the P_aguB-gfp fusion and the Cm^r	[33]
pAGDIcre1	pAGDI derivative bearing the mutated P_aguB and the Cm^r	This work
pAGDIcre2	pAGDI derivative bearing the mutated P_aguB and the Cm^r	This work
pAGDIcre3	pAGDI derivative bearing the mutated P_aguB and the Cm^r	This work
pACE	Vector for ACE system containing the P_aguR-aguR-P_aguB cassette from pAGDIcre3 and the Cm^r	This work
pACE-gfp	pACE derivative harbouring the gfp gene from pAG2 and the Cm^r	This work
pNZ8048-pep	pNZ8048 derivative harbouring the pep gene from pUC57-PEPand the Cm^r	This work
pACE-pep	pACE derivative harbouring the pep gene from pNZ8048-PEP and the Cm^r	This work

P_aguR, aguR promoter; P_aguB, aguB promoter; pep, prolyl endopeptidase gene; Cm^r, chloramphenicol resistance marker; Ap^r, ampicillin resistance marker

ISSN: 1475-2859