Fig. 2

Generation of mutations in the cre site of P _aguB in the AGDI cluster of L. lactis CECT 8666, and their effect on promoter activity. a Genetic detail of the different gfp fusions made with the wild type P _aguB region (fusion pAGDI) and the derived promoters carrying different mutations in the cre site (fusions pAGDIcre1, pAGDIcre2 and pAGDIcre3). The introduced mutations are highlighted in black. Dashed lines indicate sequence discontinuities. b–e Promoter activity reported by GFP fluorescence (arbitrary units) for the wild type P _aguB and mutants assayed in the presence of 30 and 120 mM glucose under induction by 20 mM agmatine. Data represent the average of three independent experiments. Bars indicate standard deviations (*p < 0.05)