Fig. 6

Biotransformation of 1,3PDO to 3HP. Batch biotransformation of 1,3PDO (filled square) to 3HP (filled triangle) via 3HPA (filled diamond) as intermediate using resting cells of G. oxydans (3.25 g_CDW/L). a Model solution containing 10 g/L 1,3PDO and b 1,3PDO (10 g/L) in mixture with 3HP (11.8 g/L) obtained from glycerol biotransformation using resting cells of L. reuteri. The biotransformation was done in 3 L bioreactor with 1 L working volume. Temperature was controlled at 28 °C, stirrer speed at 800 rpm, airflow at 1 v/v/m, and pH at 5.5 through addition of 5 N NH₄OH during the whole biotransformation process