Fig. 4

Effect of B. bifidum S17/pAO-S0_CD and 5-FC on HT-29/EGFP4 MCTS. a HT-29/EGFP4 MCTS were incubated with B. bifidum S17/pAO-S0_CD (S17 CD) alone or in combination with 0.25 mg/mL 5-FC up to for 96 h. As controls, MCTS were treated with 200 nM staurosporine (stauro), 5-FC, or medium alone (-). b HT-29/EGFP4 MCTS were grown in the presence and absence of 0.5 or 0.05 mg/mL 5-FU. All MCTS were analyzed for growth by fluorescence measurements for 96 h. Fluorescence intensities were normalized to give 100 % for each MCTS at the start of the experiment (t = 0 h). Values are relative fluorescent units (RFU) per MCTS and are mean ± SEM of three MCTS. Results of one representative of three independent experiments are shown. Statistical analysis was performed using two-way ANOVA with Bonferroni post-test correction for multiple comparisons (***difference to untreated MCTS significant with adjusted P < 0.001, all other comparisons not significant)