Table 2 Escherichia coli strains and plasmids used in this study

E. coli WG1

E. coli K-12 wild type strain

CGSC#: 5073

E. coli MG1655

F-, λ-, rph-1

CGSC#: 7740

E. coli MG1655*

F-, λ ⁻, rph-1, rpoS396(Am), see Table 1

CGSC#: 7740/this study

E. coli MG-rph⁺

MG1655-derivative Nuc5⁻.dnaG.Q576A, rph ⁺

[60]/this study

E. coli Seq⁺ (KRE10000)

MG1655 rph ⁺

Obtained from Donald Court, [23, 75]

E. coli BB

E.coli B wild type strain

CGSC#: 2507

E. coli REL606

F-, tsx-467 (Am), araA230, lon, rpsL227 (strR), hsdR-, [mal ⁺ ] _K-12 (λ^S)

CGSC#: 12149

E. coli W

E.coli W wild type strain

ATCC: 9637 [29]

E. coli Mach1

Commercially available cloning host derived from E. coli W

Invitrogen™

E. coli Nuc5.dnaG.Q576A.tolC-

MG1655-derived MAGE strain used for markerless genome engineering

[76], Addgene strain #41699

pAC-LYC04

cat, idi from H. pluvialis, crtEIB from P. agglomerans

[77]

pTrc99SS

bla, P_trc, truncated MCS (derived from pTrc99a)

[78]/this study

pT-dxs

bla, P_trc, optimized dxs from P. trichocarpa

This study

pT-ispS (L70R)

bla, P_trc, optimized ispS from P. alba

This study

pRpoS

kan, P_rpoS, rpoS from MG1655 (derived from pET28a)

This study

pAra-KatE

bla, P_araBAD, katE from E. coli MG1655 (derived from pKD46)

This study/[79]