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Table 1 Summary of process parameters and results

From: A novel one-step expression and immobilization method for the production of biocatalytic preparations

  Plasmid Expression duration, h Expression temperature, °C CDW concentration prior to lysis, g/L Activity per dry weight cellular envelopes, U/g
Fed-batch pCOLADuet 18 25 8.5 ± 0.3 312 ± 29
Batch pCOLADuet 3 35 4.3 ± 0.3 632 ± 250
Batch pET28a 3 35 3.9 ± 0.2 753 ± 190
  1. Expression was chemically induced with 0.1 mM IPTG after biomass formation in a batch phase at 35 °C
  2. CDW cell dry weight