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Table 1 Summary of process parameters and results

From: A novel one-step expression and immobilization method for the production of biocatalytic preparations

 

Plasmid

Expression duration, h

Expression temperature, °C

CDW concentration prior to lysis, g/L

Activity per dry weight cellular envelopes, U/g

Fed-batch

pCOLADuet

18

25

8.5 ± 0.3

312 ± 29

Batch

pCOLADuet

3

35

4.3 ± 0.3

632 ± 250

Batch

pET28a

3

35

3.9 ± 0.2

753 ± 190

  1. Expression was chemically induced with 0.1 mM IPTG after biomass formation in a batch phase at 35 °C
  2. CDW cell dry weight
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Microbial Cell Factories

ISSN: 1475-2859

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