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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A novel one-step expression and immobilization method for the production of biocatalytic preparations

Fig. 1

Schematic display of the new technique using E mediated lysis. 1 Expression of β-galactosidase as a model enzyme with C-terminal membrane anchor from cytochrome b5 (rabbit liver), posttranslational tetramer formation and inner membrane insertion. 2 Expression of lytic phage protein E and insertion into the cell membranes. 3 Pore formation by protein E and lysis with release of the cytosol. 4 Cellular envelope with immobilized enzymes and lysis pore

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