Fig. 1

Schematic display of the new technique using E mediated lysis. 1 Expression of β-galactosidase as a model enzyme with C-terminal membrane anchor from cytochrome b₅ (rabbit liver), posttranslational tetramer formation and inner membrane insertion. 2 Expression of lytic phage protein E and insertion into the cell membranes. 3 Pore formation by protein E and lysis with release of the cytosol. 4 Cellular envelope with immobilized enzymes and lysis pore