Table 2 Strains and plasmids used in this study

Strains

 E. coli DH5α

Coli genetic stock center strain (CGSC) No. 12384

CGSC^a

 E. coli JM109

recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac proAB)/F’ [traD36, proAB ⁺, lac ^q lacZΔM15]

TaKaRa (Dalian, China)

 TB01

JM109, P _kbl-tdh ::P _trc

This study

 TB02

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc

This study

 TB03

JM109, ΔserB

This study

 TB04

JM109, P _kbl-tdh ::P _trc , ΔserB

This study

 TB05

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , ΔserB

This study

 TB06

JM109, thrA(C1034T), P _thrABC ::P_Trc-162

 TB07

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162

This study

 TB09

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162, P _ppc ::P _tac

This study

 TB13

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162, P _ppc ::P _tac , P _glyA ::P _Trc-162

This study

 TB14

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162, P _ppc ::P _tac , P _gcv ::P _Trc-162

This study

 TB15

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162, P _ppc ::P _tac , P _glyA ::P _trc , P _gcvA ::P _Trc-162

This study

 TB17

JM109, P _kbl-tdh ::P _trc , P _sdaA ::P _trc , thrA(C1034T), P _thrABC ::P_Trc-162, P _ppc ::P _tac , P _glyA ::P _Trc-162, P _pntAB ::P _Trc-162

This study

Plasmids

This study

 pBHR68

pBluscript SK(−) derivative, phbA _Re , phbB _Re , phbC _Re cloned from R. eutropha

[8]

 pTKRED

pSC101 replication, temperature sensitive replication origin, Spc ^r, P _araBAD -driven I-SceI gene, Red recombinase expression plasmid, lac-inducible expression

[40]

 pTKS/CS

p15A replication, Cm ^r, Tet ^r, I-SceI restriction sites

[40]