Table 3 Strains and plasmids used in this study
Strains and plasmids | Genotype | Reference |
|---|---|---|
Escherichia coli strains | ||
JM109 | F’(traD36, proAB+, lacIq, Δ(lacZ)M15) endA1 recA1 hsdR17 (r− K, m+ K) mcrA supE44 λ− gyrA96 relA1 Δ(lac-proAB) thi −1 | Takara |
JM109(ΔyfgM) | Same as JM109, but with ΔyfgM | This study |
JM109(ΔsdhB) | Same as JM109, but with ΔsdhB | This study |
JM109(ΔgapA) | Same as JM109, but with ΔgapA | This study |
JM109(ΔpepB) | Same as JM109, but with ΔpepB | This study |
JM109(Δbcp) | Same as JM109, but with Δbcp | This study |
JM109(ΔdppA) | Same as JM109, but with ΔdppA | This study |
JM109(ΔyfgM)/yfgM | Same as JM109, but with ΔyfgM/pQE-yfgM | This study |
JM109(ΔsdhB)/sdhB | Same as JM109, but with ΔsdhB/pQE-sdhB | This study |
JM109(ΔgapA)/gapA | Same as JM109, but with ΔgapA/gapA | This study |
JM109(ΔpepB)/pepB | Same as JM109, but with ΔpepB/pQE-pepB | This study |
Plasmids | ||
pKD13, pKD46, and pCP20 | Expression vector | 34 |
pQE80L | Expression vector | QIAGEN |
pHACM-rpoD WT | Plasmid harboring WT rpoD | 22 |
pHACM-rpoD C9 | Plasmid harboring rpoD mutant C9 | This study |