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Table 3 Strains and plasmids used in this study

From: Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering

Strains and plasmids Genotype Reference
Escherichia coli strains
 JM109 F’(traD36, proAB+, lacIq, Δ(lacZ)M15) endA1 recA1 hsdR17 (r K, m+ K) mcrA supE44 λ gyrA96 relA1 Δ(lac-proAB) thi −1 Takara
 JM109(ΔyfgM) Same as JM109, but with ΔyfgM This study
 JM109(ΔsdhB) Same as JM109, but with ΔsdhB This study
 JM109(ΔgapA) Same as JM109, but with ΔgapA This study
 JM109(ΔpepB) Same as JM109, but with ΔpepB This study
 JM109(Δbcp) Same as JM109, but with Δbcp This study
 JM109(ΔdppA) Same as JM109, but with ΔdppA This study
 JM109(ΔyfgM)/yfgM Same as JM109, but with ΔyfgM/pQE-yfgM This study
 JM109(ΔsdhB)/sdhB Same as JM109, but with ΔsdhB/pQE-sdhB This study
 JM109(ΔgapA)/gapA Same as JM109, but with ΔgapA/gapA This study
 JM109(ΔpepB)/pepB Same as JM109, but with ΔpepB/pQE-pepB This study
Plasmids
 pKD13, pKD46, and pCP20 Expression vector 34
 pQE80L Expression vector QIAGEN
 pHACM-rpoD WT Plasmid harboring WT rpoD 22
 pHACM-rpoD C9 Plasmid harboring rpoD mutant C9 This study