Secretion of biologically active TSLP by recombinant Lactococcus lactis.
a Schematic representation of the expression cassette: GroESL promoter (pGroESL) followed by tslp gene, flanked by the signal peptide (SP) of Exp4 and a terminator (Ter). b Detection of TSLP by ELISA in supernatant fractions from NaCl-induced LL-TSLP cultures or c heat-shock-induced LL-TSLP cultures. d Detection of IL-12 by ELISA in LPS-induced-BMDCs supernatants co-incubated with commercial recombinant TSLP (rTSLP) (0 or 10 ng/mL), recombinant TSLP produced by LL-TSLP cultures (concentrated TSLP) (10 ng/mL) or with equivalent amount of protein from concentrated supernatant LL-NUC cultures (negative control). Statistically significant differences (*P < 0.05, ***P < 0.001)