Table 2 Engineering the ALA production in C. glutamicum using the gltX, hemA and hemL from different sources
From: Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose
C. glutamicum strains | Expressed genes | Cell biomass (OD600) | ALA (mg/L) | Lactate (g/L) | Acetate (g/L) | PBG (mg/L) | Heme (mg/L) |
|---|---|---|---|---|---|---|---|
PECX | – | 18.84 ± 0.76 | 25.44 ± 1.56 | 7.45 ± 0.46 | 32.41 ± 1.27 | 1.26 ± 0.26 | 0.12 ± 0.05 |
CEAL | hemA (C. glutamicum) and hemL (E. coli) | 18.07 ± 0.84 | 128.13 ± 8.94 | 4.25 ± 0.19 | 26.24 ± 2.44 | 21.34 ± 2.47 | 1.54 ± 0.28 |
SCAL | hemA M (S. arizona) and hemL (C. glutamicum) | 17.75 ± 0.72 | 83.47 ± 7.33 | 0.19 ± 0.07 | 4.23 ± 0.59 | 11.26 ± 1.24 | 0.98 ± 0.18 |
SEAL | hemA M (S. arizona) and hemL (E. coli) | 18.33 ± 0.88 | 425.11 ± 15.69 | 0.04 ± 0.008 | 0.28 ± 0.03 | 97.87 ± 4.02 | 3.18 ± 0.54 |
PSEC | hemA M (S. arizona), hemL (E. coli) and gltX (C. glutamicum) | 16.81 ± 0.74 | 251.36 ± 10.11 | 0.17 ± 0.03 | 1.45 ± 0.12 | 117.92 ± 2.49 | 4.15 ± 0.75 |
PSEE | hemA M (S. arizona), hemL (E. coli) and gltX (E. coli) | 16.45 ± 0.78 | 276.21 ± 9.26 | 0.14 ± 0.05 | 1.12 ± 0.34 | 121.38 ± 2.53 | 4.22 ± 0.62 |