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Table 1 Effect of NADH concentration and presence of NaMN on levels of DMBI and α-RP in reactions with the BluB/CobT2

From: BluB/CobT2 fusion enzyme activity reveals mechanisms responsible for production of active form of vitamin B12 by Propionibacterium freudenreichii

NADH (mM)

FMN (100 µM)

FMN (100 µM) + NaMN (200 µM)

DMBI (nM)

α-RP (nM)

DMBI (nM)

0

120 ± 6

–

–

20

1362 ± 12

4458 ± 200

0.5 ± 0.2

40

2301 ± 9

5095 ± 215

2 ± 0.5

  1. The concentrations of DMBI and α-RP were measured following incubation of reactions for 48 h
  2. The values reported are an average of three independent reactions, with standard deviation
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Microbial Cell Factories

ISSN: 1475-2859

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