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Fig. 4 | Microbial Cell Factories

Fig. 4

From: BluB/CobT2 fusion enzyme activity reveals mechanisms responsible for production of active form of vitamin B12 by Propionibacterium freudenreichii

Fig. 4

Production and phosphoribosylation (activation) of DMBI by BluB/CobT2. a The LC chromatogram of the DMBI synthesis reaction containing BluB/CobT2, FMN and NADH after incubation for 48 h. b The LC chromatogram of two-step DMBI synthesis and activation reaction producing α-RP in the presence of the BluB/CobT2, FMN, NADH and NaMN after incubation for 48 h. The peaks were verified by MS/MS analysis. See also Additional file 1: Figure S1

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Microbial Cell Factories

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