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Table 4 Glucose (Glc) consumption rate (GCR), molar yield and productivity of 2,3-butanediol (2,3-BD), and carbon recovery (CR) achieved with C. glutamicum ΔaceEΔpqoΔldhA(pEKEx2-als,aldB, Ptuf butA) and C. glutamicum ΔaceEΔpqoΔldhAΔmdh(pEKEx2-als,aldB, Ptuf butA) strains under different aeration conditions

From: Engineering Corynebacterium glutamicum for the production of 2,3-butanediol

  Airflow (mL min−1) GCR (nmol min−1  mg CDW−1) Yield (mol 2,3-BD per mol Glc) Productivity (nmol min−1 mg CDW−1) CR (%)
ΔaceEΔpqoΔldhA (pEKEx2-als,aldB, Ptuf butA) 5 7.2 ± 0.6 0.44 ± 0.04 3.4 ± 0.3 89 ± 6
10 15.8 ± 1.7 0.57 ± 0.03 8.1 ± 0.4 91 ± 4
20 14.7 ± 1.1 0.36 ± 0.06 5.0 ± 0.9 83 ± 10
ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA) 5 11.1 ± 1.0 0.52 ± 0.03 5.5 ± 0.7 85 ± 2
10 21.1 ± 0.8 0.66 ± 0.01 10.9 ± 1.8 90 ± 8
20 22.8 ± 1.6 0.46 ± 0.02 8.0 ± 0.7 78 ± 7
  1. Cells were grown aerobically on 1 % (wt/vol) potassium acetate. The second, production phase was performed in a 80-mL fermenter at 30 °C for 30 h with glucose as substrate. The cell suspension was sparged with air at the flow rates indicated. Meso-2,3-BD was by far the major stereoisomer. At the optimal aeration rate (10 mL min−1), there was 95 % of the meso-form. Values are averages of three independent experiments; a single NMR spectrum was acquired for each sample