Table 1 Strains and plasmids used in this study

Plasmids

 pUC-CCL3Gag

Amp^r; pUC57 vector with synthetic ccl3gag gene

Genescript

 pEV

Ery^r; control plasmid (“empty vector”)

[36]

 pLp_1261Inv

Ery^r; pLp_2588sAmyA [51] derivative, encoding a lipoanchor sequence from lp_1261 fused to a fragment of an invasin encoding gene; for anchoring through an N-terminal lipobox.

[36]

 pLp_1452Inv

Ery^r; as pLp_1261Inv, but with the lipoanchor from Lp_1452.

[36]

 pLp_3014Inv

Ery^r; pLp_2588sAmyA [51] derivative, encoding Lp_3014 fused to the a fragment of an invasin encoding gene; for anchoring through an N-terminal LysM domain.

[36]

 pLp_1568InvS

Ery^r; pLp_2588sAmyA [51] derivative, encoding Lp_1568 fused to a fragment of an invasion encoding gene; for N-terminal anchoring through a signal peptide-like transmembrane helix.

[36]

 pLp_0373sOFAcwa2

Ery^r; pLp_0373sNuc [51] derivative, encoding signal peptide sequence from Lp_0373 fused to the ofa gene and a subsequent anchor encoding sequence (cwa2); for C-terminal covalent anchoring to the cell wall.

[42]

 pLp_1261_CCL3Gag

Ery^r, pLp_1261Inv derivative, where inv gene fragment has been replaced by the ccl3gag fusion gene.

This study

 pLp_1452_CCL3Gag

Ery^r, pLp_1452Inv derivative, where inv gene fragment has been replaced by the ccl3gag fusion gene.

This study

 pLp_3014_CCL3Gag

Ery^r, pLp_3014Inv derivative, where inv gene fragment has been replaced by the ccl3gag fusion gene.

This study

 pLp_1586_CCL3Gag

Ery^r, pLp_1568InvS derivative, where inv gene fragment has been replaced by the ccl3gag fusion gene

This study

 pLp_CCL3Gag_Cwa

Ery^r, pLp_0373sOFAcwa2 derivative, where the ofa gene has been replaced by the ccl3gag fusion gene

This study

Strains

 L. plantarum NC8

Host strain

[55]

 E. coli TOP10

Host strain

Invitrogen

 Lp_1261CCL3Gag

L. plantarum NC8 harboring pLp_1261CCL3Gag; for surface display of the CCL3Gag protein using an N-terminal lipo-anchor

This study

 Lp_1452CCL3Gag

L. plantarum NC8 carrying pLp_1452CCL3Gag; for surface display of the CCL3Gag protein using an N-termina lipo-anchor

This study

 Lp_3014CCL3Gag

L. plantarum NC8 carrying pLp_3014CCL3Gag; for surface display of the CCL3Gag protein using an N-terminal LysM domain

This study

 Lp_1568CCL3Gag

L. plantarum NC8 carrying pLp_1568CCL3Gag; for surface display of the CCL3Gag protein using an N-terminal transmembrane anchor

This study

 Lp_CCL3Gag_Cwa

L. plantarum NC8 carrying pLp_CCL3Gag_Cwa; for surface display of the CCL3Gag protein using a C-terminal covalent cell wall anchor (Cwa)

This study

 Lp_Ev

L. plantarum NC8 carrying pEV (emtpy vector); used as a negative control strain

[36]