Table 4 Primers used in this study
Primers | Sequence (5′-3′) |
|---|---|
Primers for gene disruption and complementation | |
LwblA-F | AAGCTTTCGGGTACGCCATCTCGTA |
LwblA-R | TCTAGAGCTGCTCCCTGAACGAACA |
RwblA-F | GGATCCACGACGAGGTGTACGAGAAC |
RwblA-R | GATATCTGACGCTGCTGGAGGAGAT |
Kan-F | TCTAGAGATCCCCTGGATACCGCTCG |
Kan-R | GGATCCGTACCCGAACCCCAGAGTC |
wblAJ-F | AACTGGCGGCGGTGAATA |
wblAJ-R | ACGGACGGAGCACATATAGG |
CwblA-F | GGATCCGCCTGAACGGACGGAGCACATA |
CwblA-R | TCTAGAAGCACACTGACACCGAGGAACTTGGC |
Primers for qRT-PCR | |
RTsanG-F | GGCGTACACAGCTCAAGAGC |
RTsanG-R | AATTCGTCGATGAGCTGATC |
RTsanN-F | AGATCATGCGCTCGGACTGT |
RTsanN-R | TGGCGTGCAGGATCGGTA |
RTsanO-F | ACTGCGATCCGTGGTCAA |
RTsanO-R | TGTACTCCAGGCACTCCC |
RTsanF-F | CGGCGCTGGAGGAACGTAC |
RTsanF-R | GGGTGTAGAGGCCGATGCT |
RThrdB-F | GCTGGCCAAGGAACTCGACAT |
RThrdB-R | CGAAGCGCATGGAGACGACG |