Fig. 6
From: High-level secretion of native recombinant human calreticulin in yeast
Cellular proliferation induced by native and yeast-derived CRTs. Native CRT isolated from human placenta, and recombinant human CRT purified from yeast P. pastoris and S. cerevisiae were incubated with human fibroblasts and the assay performed as described in “Methods”. Cellular proliferation was compared to the untreated control of cells incubated in 0.5 % FBS medium without CRT. Experiments were performed in triplicate (n = 6) and error bars represent standard error of the mean (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001; Dunnett’s test for statistical significance)