Fig. 4
Comparison of G. oxydans Δupp cultivations using the BioLector microtiter plate and the RAMOS shake flask system. Online monitoring of the oxygen transfer rate (OTR) in a RAMOS shake flask and of the dissolved oxygen tension (DOT) measured via dispersed oxygen-sensitive nanoparticles in a MTP (a). Scattered light curves of cultivations with and without dispersed oxygen-sensitive nanoparticles in a MTP in the BioLector system (b). Cultivation conditions: BioLector: 48well Round Well Plate without optodes, VL = 800 µL, n = 1000 rpm, d0 = 3 mm, 30 °C; RAMOS: 250 mL-RAMOS shake flask, VL = 10 mL, n = 350 rpm, d0 = 50 mm, 30 °C; complex mannitol medium. Based on the measured DOT in MTP the OTR was calculated for the MTP with a fitted kLa-value of 186 h−1 according to Eq. 2. For a better comparison of the propagation of the scattered light signals, two specifically adjusted y axes were used