Table 4 Primers and PCR products used in this study
Primer or PCR product | Nucleotide sequence (5′–3′) or description | Amplification |
|---|---|---|
Primers | ||
JJ3-F | ACCACTCATAGTGGAAAATATTATGG | AI |
JJ5-R | GGCGGAGCTCTCCAGGCATTAAAATTGAGATTTATCTCCATAATC | AI, UA, UAI |
USPNC-F | GAATTCTCACCATGGGAAAAAAAAAGATTATCTCAGCTATTTTAATGTCTAC | UA, UAI |
JJ8-R | CCATAATATTTTCCACTATGAGTGGTAGCGTAAACACCTGACAACGG | UA |
PCR products | ||
AI | 475-bp XhoI fragment containing the mature enterocin A (entA) and immunity (entiA) genes | |
UA | 124-pb NcoI fragment containing the usp45 signal peptide (SP usp45 ) and the begining of mature entA | |
UAI | 567-bp NcoI/XhoI fragment containing the SP usp45 fused to mature entA and entiA | |