Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch

Strains/plasmid Relevant characteristics References/source
E. coli strains
 BW25113 lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78  
 JW0336 BW25113 ΔlacI::Kanr [28]
 JW3897 BW25113 ΔglpK::Kanr [28]
 JW3890 BW25113 ΔtpiA::Kanr [28]
 JW2978 BW25113 ΔyqhD::Kanr [28]
 TA1015 JW0336 removed Kanr This study
 TA2463 TA1015/pTA216, pTA1065, pTA1196 This study
 TA2207 TA1015 ΔglpK This study
 TA2660 TA2207/pTA216, pTA1196, pTA1277 This study
 TA2791 TA1015 ΔtpiA This study
 TA2945 TA2791/pTA216, pTA1196, pTA1393 This study
 TA367 BW25113 ΔgapA, Kanr Gifted by Dr. Nakahigashi
 TA386 TA367 removed Kanr This study
 TA2732 TA386/pTA216, pTA1335 This study
 TA2793 TA2732 ΔlacI/pTA216, pTA1335, Kanr This study
 TA2814 TA2793 removed Kanr This study
 TA2821 TA2814/pTA1196 This study
 TA3125 TA2814 ΔyqhD/pTA216, pTA1335 This study
 TA3130 TA3125/pTA1196 This study
Plasmids
 pCP20 Ampr, Cmr, FLP+, λcI857+, Repts [29]
 pTA216 pSC101*, Cmr, P qlacI ::lacI [15]
 pTA867 ColE1, Kanr, PllacO1::dhaB1, dhaB2, dhaB3, gdrA, gdrB, araE [24]
 pTA958 p15A, Kanr, PllacO1::tetR, PltetO1::gapA.LAA This study
 pTA1065 p15A, Specr, PllacO1::tetR, PltetO1::MCS This study
 pTA1196 ColE1, Kanr, PllacO1::dhaB1, dhaB2, dhaB3, gdrA, gdrB PllacO1::araE This study
 pTA1277 p15A, Specr, PllacO1::tetR, PltetO1::gltA.LAA This study
 pTA1393 p15A, Specr, PllacO1::tetR, PltetO1::tpiA.LAA This study
 pTA1355 p15A, Specr, PllacO1::tetR, PltetO1::gapA.LAA This study