Table 1 Bacterial strains and plasmids used in this study
From: Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch
Strains/plasmid | Relevant characteristics | References/source |
|---|---|---|
E. coli strains | ||
 BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 |  |
 JW0336 | BW25113 ΔlacI::Kanr | [28] |
 JW3897 | BW25113 ΔglpK::Kanr | [28] |
 JW3890 | BW25113 ΔtpiA::Kanr | [28] |
 JW2978 | BW25113 ΔyqhD::Kanr | [28] |
 TA1015 | JW0336 removed Kanr | This study |
 TA2463 | TA1015/pTA216, pTA1065, pTA1196 | This study |
 TA2207 | TA1015 ΔglpK | This study |
 TA2660 | TA2207/pTA216, pTA1196, pTA1277 | This study |
 TA2791 | TA1015 ΔtpiA | This study |
 TA2945 | TA2791/pTA216, pTA1196, pTA1393 | This study |
 TA367 | BW25113 ΔgapA, Kanr | Gifted by Dr. Nakahigashi |
 TA386 | TA367 removed Kanr | This study |
 TA2732 | TA386/pTA216, pTA1335 | This study |
 TA2793 | TA2732 ΔlacI/pTA216, pTA1335, Kanr | This study |
 TA2814 | TA2793 removed Kanr | This study |
 TA2821 | TA2814/pTA1196 | This study |
 TA3125 | TA2814 ΔyqhD/pTA216, pTA1335 | This study |
 TA3130 | TA3125/pTA1196 | This study |
Plasmids | ||
 pCP20 | Ampr, Cmr, FLP+, λcI857+, Repts | [29] |
 pTA216 | pSC101*, Cmr, P qlacI ::lacI | [15] |
 pTA867 | ColE1, Kanr, PllacO1::dhaB1, dhaB2, dhaB3, gdrA, gdrB, araE | [24] |
 pTA958 | p15A, Kanr, PllacO1::tetR, PltetO1::gapA.LAA | This study |
 pTA1065 | p15A, Specr, PllacO1::tetR, PltetO1::MCS | This study |
 pTA1196 | ColE1, Kanr, PllacO1::dhaB1, dhaB2, dhaB3, gdrA, gdrB PllacO1::araE | This study |
 pTA1277 | p15A, Specr, PllacO1::tetR, PltetO1::gltA.LAA | This study |
 pTA1393 | p15A, Specr, PllacO1::tetR, PltetO1::tpiA.LAA | This study |
 pTA1355 | p15A, Specr, PllacO1::tetR, PltetO1::gapA.LAA | This study |