Table 1 Strains and plasmids used in this study

Strains

 E. coli JM109

recA1, endA1, gyrA96, thi-1, hsdR17(r ^-_k m ⁻_k ), e14⁻(mcrA ⁻), supE44, relA1, Δ(lac-proAB)/F′ [traD36, proAB ⁺, lacI ^q, lacZΔM15]

B. subtilis

 168

trpC2

 WB600

(168) ΔnprE, Δ aprA, Δepr, Δ bpr, Δmpr, ΔnprB

[40]

 WB800

(168) ΔnprE, Δ aprA, Δepr, Δ bpr, Δmpr, ΔnprB, Δvpr, ΔwprA

[41]

 BSG1681

(168) ΔP_srfA

This study

 BSG101

168, pBSG03 (P_srfA-gfp)

This study

 BSG102

168, pBSG04 (P_HpaII-gfp)

This study

 BSG103

BSG1681, pBSG03 (P_srfA-gfp)

This study

 BSG104

WB600, pBSG03 (P_srfA-gfp)

This study

 BSG105

WB800, pBSG03 (P_srfA-gfp)

This study

 BSG106

168, pBSG05 (^mutP_srfA-gfp)^a

This study

 BSG107

168, pBSG06 (^mutP_srfA-ap)

This study

Plasmids

 P7Z6

zeo ^r bla ^r Cre/lox

[38]

 pUC19

pUC origin P _lac Ap ^r

TaKaRa

 pMA09

E. coli–B. subtilis shuttle vector, Ap ^r Kan ^r P_HpaII

[30]

 pMA05

E. coli–B. subtilis shuttle vector, Ap ^r, Kan ^r, P_HpaII- AP

[30]

 pBSG01

bla ^r, neo ^r, E. coli–B. subtilis shuttle vector

This work

 pBSG02

pBSG01 ligated with P_srfA

This study

 pBSG03

pBSG02 with GFP ligated downstream of P_srfA

This study

 pBSG04

pBSG03 with P_srfA replaced by P_HpaII

This study

 pBSG05

pBSG03 with ^mutP_srfA

This study

 pBSG06

pBSG02 with AP ligated downstream of ^mutP_srfA

This study