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Table 3 Number of charged residues, calculated/measured isoelectric point, and Tm for sdAb

From: Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond

Clone

Negative

Positive

pI (calc)a

pI (IEF)b

L1-G2+

10

13

8.8

>10.7

L1-G2+neg

13

13

7.0

8.3

L1-G2+neg2

13

12

6.6

7.7

A3+

12

12

7.0

8.2

A3+neg

14

11

6.0

6.8

AC+

11

11

7.2

8.2

AC+neg

12

11

6.6

7.7

AC+neg2

13

11

6.3

7.2

  1. aCalculated using ExPASy ProtParam tool
  2. bMeasured by IEF see Additional file 2: Figure S2
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Microbial Cell Factories

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