Fig. 3

Retention of sdAb binding activity and solubility following heating. SdAb at a concentration of 1 mg/ml were heated for one hour at the indicated temperature. a The percent of binding activity retained was determined by initial on rates ascertained by SPR. The binding rate of unheated sdAb was set to 100 %. b Samples were spun and the OD at 280 nm was compared to unheated samples to determine the percent soluble protein. These are plots of representative data, the same trends were seen on replication