A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

Table 2 Premedrol yield of different systems for whole-cell biotransformations performed with bCYP21A2 and various redox chains

Redox system	Overall yield premedrol (mg L⁻¹ d⁻¹)	Initial rate (mg L⁻¹ h⁻¹)
CPR	87 ± 5	13 ± 2
AdR/Adx	41 ± 6	3 ± 0.2
arh1/Adx	156 ± 2	36 ± 1
Fpr/Adx	127 ± 7	33 ± 2
arh1/etp1^fd	167 ± 5	67 ± 4
Fpr/etp1^fd	115 ± 1	31 ± 0.4

The premedrol overall yield was determined via HPLC analysis after 24 h conversion with all constructs simultaneously. The initial rate was calculated taking the first 2 h into account after starting the reaction by substrate addition. All values represent the mean of triplicates with the respective standard deviation

ISSN: 1475-2859