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Table 2 Premedrol yield of different systems for whole-cell biotransformations performed with bCYP21A2 and various redox chains

From: A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

Redox system Overall yield premedrol (mg L−1 d−1) Initial rate (mg L−1 h−1)
CPR 87 ± 5 13 ± 2
AdR/Adx 41 ± 6 3 ± 0.2
arh1/Adx 156 ± 2 36 ± 1
Fpr/Adx 127 ± 7 33 ± 2
arh1/etp1fd 167 ± 5 67 ± 4
Fpr/etp1fd 115 ± 1 31 ± 0.4
  1. The premedrol overall yield was determined via HPLC analysis after 24 h conversion with all constructs simultaneously. The initial rate was calculated taking the first 2 h into account after starting the reaction by substrate addition. All values represent the mean of triplicates with the respective standard deviation