Fig. 4

Sequence and structural comparison of the Pma1p orthologs in S. cerevisiae and P. anomala. The P. anomala plasma membrane H⁺-ATPase (PMA1_Pichia) and the S. cerevisiae plasma membrane H⁺-ATPase (PMA1_Sacc) are highly conserved with 84 % sequence identity (a). Predicted secondary structure is depicted below the alignment (red represents the α-helix and the β-sheet is shown in green). The conservation scores are plotted as bar plot below the secondary structure prediction line for each alignment column. The predicted 3D structures are compared and displayed as yellow for PMA1_Pichia and blue for PMA1_Sacc (b). The statistics of the predicted structures and corresponding comparison are shown in the Additional file 10: Table S3)