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Table 1 Kinetic parameters and carbon recovery for A. niger DS03043 and CBS513.88

From: Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger

 

A. niger DS03043

A. niger CBS513.88

A

 μ (h−1)

0.14 ± 0.01

0.10 ± 0.01

 qS (mmol/gDCW h)

1.83 ± 0.01

1.46 ± 0.01

 qGA (μmol/gDCW h)a

0.40 ± 0.03

0.05 ± 0.00

 qCIT (mmol/gDCW h)

0.02 ± 0.00

0.11 ± 0.01

 qOA (mmol/gDCW h)

0.11 ± 0.01

0.35 ± 0.02

 qO2 (mmol/gDCW h)

2.94 ± 0.03

2.67 ± 0.02

 qCO2 (mmol/gDCW h)

3.21 ± 0.02

2.79 ± 0.05

B

 YX/S (mmol/mmol)

0.49 ± 0.03

0.47 ± 0.02

 YGA/S (mmol/mmol)

0.12 ± 0.01

0.02 ± 0.00

 YCIT/S (mmol/mmol)

0.01 ± 0.00

0.07 ± 0.01

 YOA/S (mmol/mmol)

0.02 ± 0.00

0.07 ± 0.01

 YCO2/S (mmol/mmol)

0.30 ± 0.02

0.32 ± 0.03

 Carbon recovery

94 %

95 %

  1. All parameters were measured based on at least triplicate measurements. The closeness to 100 % of carbon recovery indicates that almost all carbon compounds produced have been considered
  2. aqGA: Specific production rate of glucoamylase. For the calculation of qGA, 1 mg glucoamylase equals to 50 units of enzyme activity