Fig. 2

Production of YidC-GFP and GltP-GFP in BL21(DE3) cells in the presence and absence of IPTG. BL21(DE3) cells harboring either a pET-based yidC-gfp or gltP-gfp expression vector were cultured in LB medium at 30 °C in the presence and absence of IPTG (final concentration 0.4 mM). Membrane protein production and biomass formation were monitored 4 and 24 h after the addition of IPTG. a YidC-GFP and GltP-GFP production levels in the cytoplasmic membrane of BL21(DE3) cells cultured in the presence and absence of IPTG were assessed by monitoring fluorescence (relative fluorescence unit, RFU) per milliliter of culture. Biomass formation was monitored by measuring the A₆₀₀. RFUs/ml per A₆₀₀ are shown in Additional file 1: Figure S1. b The production of membrane protein-GFP fusion per cell was determined using flow cytometry. Traces of cells cultured in the prescence of IPTG are in red and traces of cells cultured in the absence of IPTG are in black. Cells harvested after 4 h are represented by dotted lines and cells harvested after 24 h are represented by solid lines