Table 1 Plasmids and strains used in this study

Plasmids

pBaSP

pUC57 vector expressing BaSP from B. adolescentis under control of P22

De Bruyn et al. [45]

pGalE

pUC57 vector expressing galE from E. coli under control of P22

This study

pGalE2

pUC57 vector expressing galE2 from B. bifidum under control of P22

This study

pMUM4

pUC57 vector expressing codon optimized MUM4 from A. thaliana under control of P22

GeneArt^®

pF3GT

pUC57-Kan vector expressing codon optimized f3gt from Petunia hybrida under control of P22

GeneArt^®

pRhaGT

pUC57-Kan vector expressing codon optimized RhaGT from A. thaliana under control of P22

GeneArt^®

pBaSP/VvGT2/UgpA

pCX-Kan vector expressing BaSP, vvGT2 and ugpA under control of P22

De Bruyn et al. [45]

pGalE/F3GT/UgpA

pCX-Kan vector expressing galE, f3gt and ugpA under control of P22

This study

pGalE2/F3GT/UgpA

pCX-Kan vector expressing galE2, f3gt and ugpA under control of P22

This study

pMUM4/RhaGT/UgpA

pCX-Kan vector expressing MUM4, RhaGT and ugpA under control of P22

This study

pKD46

λ Red recombinase expression, Amp^r

Datsenko and Wanner [62]

pCP20

FLP recombinase expression, Amp^r, Cm^r

Datsenko and Wanner [62]

pKD3

Cm cassette template, Cm^r, Amp^r

Datsenko and Wanner [62]

pKD4

Kan cassette template, Kan^r, Amp^r

Datsenko and Wanner [62]

Strains

E. coli DH5α

General cloning host

Coli Genetic Stock Center

E. coli W

Escherichia coli W ATCC 9637

BCCM/LMG

E. coli W ∆cscAR

E. coli W with cscAR-deleted

De Bruyn et al. [45]

E. coli W ∆cscAR ∆melA::L4-P22-BaSP-L5

E. coli W with cscAR and melA deleted and L4-P22-BaSP-L5 integrated in the genome

This study

E. coli W ∆cscAR ∆glgC::L4-P22-BaSP-L5

E. coli W with cscAR and glgC deleted and L4-P22-BaSP-L5 integrated in the genome

This study

E. coli W ∆cscAR ∆pgm ∆agp ∆ushA ΔlacZYA::P22-lacY ΔgalETKM

E. coli W with cscAR, pgm, agp, ushA, lacZYA, melA and galETKM deleted and P22-lacY integrated in the genome

De Bruyn et al. [45]

E. coli W ∆cscAR ∆pgm ∆agp ∆ushA ΔlacZYA::P22-lacY ΔgalETKM ΔmelA::P22-BaSP (sGLYC)

E. coli W with cscAR, pgm, agp, ushA, lacZYA, melA and galETKM deleted and P22-lacY and L4-P22-BaSP-L5 integrated in the genome

This study

sGAL1

E. coli W + pF3GT

This study

sGAL2

sGLYC + pGalE/F3GT/UgpA

This study

sGAL3

sGLYC + pGalE2/F3GT/UgpA

This study

sRHA1

E. coli W + pRhaGT

This study

sRHA2

sGLYC + pMUM4 + pRhaGT

This study

sRHA3

sGLYC + pMUM4/RhaGT/UgpA

This study