Table 3 Primer sequences used for the construction of mutants
From: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy
Primer name | Primer sequence a |
|---|---|
pPIC9-F | 5’-GTGCTCAACGGCCTCAACCTACTACTG-3’ |
pPIC9-R | 5’-TAGGTTGAGGCCGTTGAGCACCGCCGC-3’ |
MPH-F | 5’-GAAAAGCGTGAAGCCGAGGCCGCTGCTCCACAAGTTAGAACT-3’ |
MPH-R | 5’-AGCTTCAGCCTCTCTTTTCTC-3’ |
CHBD-F | 5’-AAAAGAGAGGCTGAAGCTATGGCAGAACAAAGCGACAAG-3’ |
CHBD-R | 5’-CTCGGCTTCACGCTTTTCAAGGGTTTCCGAAGGCTTGGC-3’ |
GST-F | 5’-AAAAGAGAGGCTGAAGCTATGTCCCCTATACTAGGTTAT-3’ |
GST-R | 5’-CTCGGCTTCACGCTTTTCGTCACGATGCGGCCGCTCGAG-3’ |
MBP-F | 5’-AAAAGAGAGGCTGAAGCTATGAAAATAAAAACAGGTGCA-3’ |
MBP-R | 5’-CTCGGCTTCACGCTTTTCTCCGCCAAAACAGCCAAGCTG-3’ |
CBD-F | 5’-AAAAGAGAGGCTGAAGCTATGAAAATCGAAGAAGGT-3’ |
CBD-R | 5’-CTCGGCTTCACGCTTTTCTTGAAGCTGCCACAAGGC-3’ |
K284R-F | 5’-GCTGCCGTTGAGAGA CGTAAAGCTTTCGCTGATGCC-3’ |
K284R-R | 5’-TTTACG TCTCTCAACGGCAGCAGACTTGGAGTCAGA-3’ |
K285R-F | 5’-GCTGCCGTTGAGAGAAAG CGTGCTTTCGCTGATGCC-3’ |
K285R-R | 5’-ACG CTTTCTCTCAACGGCAGCAGACTTGGAGTCAGA-3’ |
KK-F | 5’-GCTGCCGTTGAGAGA CAGCGTGCTTTCGCTGATGCC-3’ |
KK-R | 5’-ACGCTG TCTCTCAACGGCAGCAGACTT-3’ |
K277D-F | 5’-TTGGACTCTGACTCC GATTCTGCTGCCGTTGAG-3’ |
K277D-R | 5’-ATC GGAGTCAGAGTCCAATTGGTT-3’ |