Table 3 List of experiments performed for MBCOMT biosynthesis from P. pastoris bioreactor methanol-induced cultures based on CCD and ANN modeling
Experiment number (ANN model iterations) | Input variables level | Output | |||
|---|---|---|---|---|---|
Methanol constant feed flow-rate (mL/L/h) | Induction temperature (°C) | DMSO concentration [%(v/v)] | Observed | Predicted | |
DoE | |||||
1 | 1 | 20 | 4 | 126.1 | 122.2 |
2 | 3 | 20 | 4 | 163.9 | 139.3 |
3 | 1 | 30 | 4 | 47.4 | 97.0 |
4 | 3 | 30 | 4 | 188.0 | 187.1 |
5 | 1 | 20 | 6 | 138.0 | 143.8 |
6 | 3 | 20 | 6 | 130.6 | 139.4 |
7 | 1 | 30 | 6 | 151.4 | 97.5 |
8 | 3 | 30 | 6 | (153.9) | 358.1 |
9 | 1 | 25 | 5 | 105.3 | 116.5 |
10 | 3 | 25 | 5 | 137.9 | 134.2 |
11 | 2 | 20 | 5 | 115.2 | 136.8 |
12 | 2 | 30 | 5 | 101.1 | 105.5 |
13 | 2 | 25 | 4 | 183.9 | 197.2 |
14 | 2 | 25 | 6 | 222.6 | 218.5 |
15 | 2 | 25 | 5 | 252.5 | 243.3 |
16 | 2 | 25 | 5 | 243.8 | 243.3 |
17 | 2 | 25 | 5 | 230.3 | 243.3 |
I | |||||
18 | 1 | 22.5 | 6 | 364.3 | 343.1 |
19 | 1 | 22.5 | 6 | 364.6 | 343.1 |
20 | 1 | 22.5 | 6 | 357.6 | 343.1 |
II | |||||
21 | 2.9 | 30 | 6 | 390.6 | 383.1 |
22 | 2.9 | 30 | 6 | 391.5 | 383.1 |
III | |||||
23 | 3 | 30 | 6 | 377.1 | 358.1 |
24 | 3 | 30 | 6 | 377.4 | 358.1 |
IV | |||||
25 | 2.5 | 30 | 6 | 263.0 | 258.9 |
26 | 2.5 | 30 | 6 | 283.7 | 258.9 |
Final validation | |||||
27 | 2.9 | 30 | 6 | – | 384.8 |