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Fig. 2 | Microbial Cell Factories

Fig. 2

From: An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures

Fig. 2

a Analysis of different methanol flow-rates (without the addition of DMSO) and different DMSO concentrations (keeping constant the methanol flow-rate at 3.6 mL/L/H) on MBCOMT specific activity (nmol/h/mg of protein) obtained by P. pastoris bioreactor cultures. b Time course analysis of the methanol levels in the above mentioned assays measured by HPLC-RID. In both experiments, a 3-h period of the glycerol fed-batch was applied and induction was conducted during 12 h (each value represents the mean of three independent samples).

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Microbial Cell Factories

ISSN: 1475-2859

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