Figure 1From: Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoterDynamic batch cultivation with substrate pulses. Glucose, sorbitol, mannose, fructose, maltose, glycerol and lactic acid were sequentially pulsed after a batch on glucose. The CER signal (continuous black line) was used to follow metabolic activity. The specific substrate uptake rate (qs, black dot) was determined for the different substrates.Back to article page