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Figure 2 | Microbial Cell Factories

Figure 2

From: Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo

Figure 2

Effect of surface expression of aDec on internalization of L. plantarum by DCs. a Monocyte derived hDCs were co-incubated with Lp-WT, Lp-Lip-aDec, Lp-CWA-aDec or Lp-LysM-aDec with or without addition of a free anti human DEC205 antibody. After incubation, the hDCs were washed and non-internalized bacteria were killed by treatment with gentamicin. The numbers of internalized bacteria were counted as CFU. Same amount of DCs was used in each experiment. b BMDCs were co-incubated with Lp-WT, Lp-Lip-aDec, Lp-CWA-aDec or Lp-LysM-aDec. After incubation, the BMDCs were washed and non-internalized bacteria were killed by treatment with gentamicin. The numbers of internalized bacteria were counted as CFU. Each point represents independent wells and results are presented as mean ± SEM. The results presented are from one experiment representative of three performed independently. Statistically significant differences are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.

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Microbial Cell Factories

ISSN: 1475-2859

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