Figure 6
Overexpression of GDH2 improves the cold performance of the QA23 ho wine yeast strain. a Ura− derivatives of the wine strain QA23 ho were transformed with YEplac195-based plasmids (URA3) containing GDH1 or GDH2 and assayed for growth at the indicated temperatures on SCD-Ura agar medium. Transformants carrying the empty plasmid were also tested (Control). In all cases, cells were pre-grown and treated as described in the Figure 2. b The same strains were tested for growth at 12°C in synthetic grape must (left graph) or liquid SCD-Ura (right graph) medium. Growth of yeast cultures was monitored by measuring the cell suspension’s optical density at 600 nm (OD600) for the indicated period. A representative experiment is shown.