Table 2 Plasmids used in this study
Plasmids | Genotype and/or relevant characteristic(s) | Source |
|---|---|---|
pET28-a(+) | E. coli expression plasmid, T7 promoter, T7 terminator, Kanr | Lab stock |
pET28L | pET28-a(+) derivative, amyl | This work |
pET28S | pET28-a(+) derivative, amys | This work |
pMA5 | E. coli/B. subtilis shuttle vector, P HpaII , Apr, Kmr | BGSC |
pMA5L | pMA5 derivative, SP amyl , amyl, T4 terminator | This work |
pMA5S | pMA5 derivative, SP amys , amys, T4 terminator | This work |
pHCMC04 | P xylA , Apr, Cmr | Lab stock |
pHT43 | P grac , SP amyQ , Apr, Cmr | Lab stock |
pDD | Integration vector, pDL derivative, Apr, Cmr | This work |
pDDX | pDD derivative, P xylA | This work |
pDDXG | pDDX derivative, P xylA , P grac | This work |
pDDXBG | pDDXG derivative, bgaB | This work |
pDDXGB | pDDXG derivative, bgaB | This work |
pDD01 | pDDXG derivative, ffh | This work |
pDD02 | pDDXG derivative, hbs | This work |
pDD03 | pDDXG derivative, scr | This work |
pDD04 | pDDXG derivative, SRP | This work |
pDD05 | pDDXG derivative, ftsy | This work |
pDD06 | pDDXG derivative, csaA | This work |
pDD07 | pDDXG derivative, secA | This work |
pDD08 | pDDXG derivative, secY | This work |
pDD09 | pDDXG derivative, secE | This work |
pDD10 | pDDXG derivative, secG | This work |
pDD11 | pDDXG derivative, secYEG | This work |
pDD12 | pDDXG derivative, secDF | This work |
pDD13 | pDDXG derivative, yrbF | This work |
pDD14 | pDDXG derivative, spoIIIJ | This work |
pDD15 | pDDXG derivative, yqjG | This work |
pDD16 | pDDXG derivative, sipT | This work |
pDD17 | pDDXG derivative, sipS | This work |
pDD18 | pDDXG derivative, sipU | This work |
pDD19 | pDDXG derivative, sipV | This work |
pDD20 | pDDXG derivative, sipW | This work |
pDD21 | pDDXG derivative, groESL operon | This work |
pDD22 | pDDXG derivative, partial dnaK operon | This work |
pDD23 | pDDXG derivative, prsA | This work |
pDD231 | pDD23 derivative, prsA, SRP | This work |
pDD232 | pDD23 derivative, prsA, ftsy | This work |
pDD233 | pDD23 derivative, prsA, secA | This work |
pDD234 | pDD23 derivative, prsA, secYEG | This work |
pDD235 | pDD23 derivative, prsA, secDF | This work |
pDD236 | pDD23 derivative, prsA, groESL operon | This work |
pDD237 | pDD23 derivative, prsA, partial dnaK operon | This work |
pDD238 | pDD23 derivative, prsA, sipT | This work |
pDD239 | pDD23 derivative, prsA, sipS | This work |