Table 3 Primers used in this study for the knockout of TRP1 and insertion of pCM vectors into yeast strains
Primers | Sequence |
|---|---|
TRP1 knockout forward | cgccagatggcagtagtggaagatattctttattgaaaaatagcttgtcaATGACAGTCAACACTAAGACCTATA |
TRP1 knockout reverse | ttttatgcttgcttttcaaaaggcctgcaggcaagtgcacaaacaatactTTATAATTGGCCAGTCTTTTTC |
pCM161 forward | ATCGATATGCTTAAAATAAAGGCC |
pCM161 reverse | GGATCCCTATTCCCTGATAGTGGC |