Figure 4

p-Coumaryl alcohol titers of all 81 constructed variants of the synthetic p-coumaryl alcohol operon. The clones are numbered (and colored) from 1 to 81 in accordance to Table 1. The blue, black and grey bars represent the SD sequence–START codon spacing of the plasmid-encoded rstal-gene, which was set to 5, 9 or 13 nt, respectively (blue 5 nt; black 9 nt; grey 13 nt). The microtiter plate screening was performed in duplicate and both product titers for each clone are displayed (first screening: broad white bar; second screening: thin colored bar inside the white bar).