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Figure 2 | Microbial Cell Factories

Figure 2

From: Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae

Figure 2

Color of the different strains constructed. a Color change from strain JLS01, which only expressed the BST1 gene from S. cerevisiae and the carotenogenic genes crtYB and crtI from X. dendrorhous, to JLS02 that additionally expressed the P. hybrida gene PhCCD1. b Color of the different strains expressing episomal versions of the crtYb/crtI/PhCCD1 genes. All the strains expressed the integrated BTS1, crtYB, crtI and Ph CCD1 genes and were subsequently transformed with episomal vectors containing crtYB (JLS3), crtI (JLS04), PhCCD1 (JLS05), crtYB-crtI (JLS06) and crtYB-PhCCD1 (JLS07). All pellets were obtained after 48 h of cultivation in a two-phase culture with 10% dodecane at 20°C. c Representative plate of the constructed strains. Cells were plated after 48 h of cultivation in two-phase culture with 10% dodecane.

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Microbial Cell Factories

ISSN: 1475-2859

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