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Figure 1 | Microbial Cell Factories

Figure 1

From: Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae

Figure 1

Engineering platform for β-ionone production in yeast. The blue arrows/genes indicate the modifications of the strain SCIGS22 constructed by Scalcinati et al. [20]. The red arrows/genes show the genes expressed in the present study. In SCIGS22, ERG20 (encoding FPP synthase) was over-expressed, DPP1 and LPP1 (encoding lipid phosphate phosphatases) were deleted and the promoter of ERG9 (encoding squalene synthase) was replaced with the HXT1 promoter. In the present study, the tHMG1 (encoding a truncated HMG-CoA reductase), and BTS1 (encoding geranylgeranyl diphosphate synthase) genes were overexpressed. The heterologous genes crtYB (encoding phytoene synthase/lycopene cyclase), crtI (encoding phytoene desaturase) and PhCCD1 (encoding carotenoid cleavage dioxigenase from P. hybrida) were both integrated and expressed episomally. HMG-CoA 3-hydroxy-3-methylglutaryl-CoA, IPP isopentenyl diphosphate, DMAPP dimethylallyl diphosphate, FPP farnesyl diphosphate, GGPP geranylgeranyl diphosphate.

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Microbial Cell Factories

ISSN: 1475-2859

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