Figure 4

Production of hydroxystyrene derivatives by wild type E. coli strains with engineered expression vectors. The productivity of 4-hydroxystyrene (blue bar) and 3,4-dihydroxystyrene (red bar) with pET-opTD and pET-opT5D in the wild type E. coli C41(DE3) strain. Error bars indicate standard errors of the means (n = 3). The code ‘ND’ indicates that something was not detected in HPLC analysis. The production of 4-hydroxystyrene, 3,4-dihydroxystyrene, 4-hydroxy-3-methoxystyrene in wild type E. coli C41(DE3) were compared with single-factor ANOVA (P < 0.05) using Microsoft Excel. A different letter code (asterisk) indicates significant difference (P = 2.38E−08).