Table 1 PHAs accumulation in the wild-type and PHA synthase defective mutants of P. pseudoalcaligenes CECT5344
Nitrogen source | Strain | CDW (g L−1) | PHAs content | |||
|---|---|---|---|---|---|---|
scl-PHAs (% CDW) | mcl-PHAs (% CDW) | mcl-PHA monomer composition (%) | ||||
OH-C6 | OH-C8 | |||||
Ammonium chloride | Wild-type | 1.18 ± 0.1 | 64.95 ± 3.60 | 20.12 ± 0.08 | 5.75 ± 0.49 | 94.25 ± 0.35 |
Mpha− | 0.76 ± 0.05 | 66.14 ± 1.95 | – | – | – | |
Spha− | 0.48 ± 0.03 | – | 36.81 ± 1.90 | 9.16 ± 0.08 | 90.84 ± 0.10 | |
Mpha−/Spha− | 0.34 ± 0.03 | – | – | – | – | |
Sodium cyanide | Wild-type | 0.22 ± 0.04 | 31.02 ± 3.25 | 16.37 ± 2.81 | 5.97 ± 0.11 | 94.03 ± 0.14 |
Mpha− | 0.28 ± 0.02 | 55.47 ± 4.29 | – | – | – | |
Spha− | 0.18 ± 0.02 | – | 15.44 ± 1.05 | 6.08 ± 0.08 | 93.92 ± 0.06 | |
Mpha−/Spha− | 0.22 ± 0.03 | – | – | – | – | |
Jewelry residue | Wild-type | 0.34 ± 0.03 | 29.29 ± 0.74 | 25.63 ± 1.27 | 5.58 ± 0.07 | 94.42 ± 0.05 |
Mpha− | 0.36 ± 0.04 | 39.92 ± 2.12 | – | – | – | |
Spha− | 0.28 ± 0.04 | – | 19.94 ± 0.02 | 5.43 ± 0.06 | 94.57 ± 0.13 | |
Mpha−/Spha− | 0.28 ± 0.05 | – | – | – | – | |