Figure 2

Bacterial growth, cyanide consumption and PHAs accumulation in a batch reactor culture of P. pseudoalcaligenes CECT5344. Experiment was conducted in a bioreactor with 12.5 mM octanoate as carbon source and 2 mM cyanide from the jewelry residue as the sole nitrogen source, under conditions described in Methods. Cell growth was monitored by estimating the absorbance at 600 nm (A₆₀₀). At the indicated times, cells were collected by centrifugation and the PHAs content was analyzed by GC–MS. The scl-PHAs content (black bars) and the mcl-PHAs content (grey bars) are given as percentage of CDW.