A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq

Table 1 Transcriptomic profiles of A. mediterranei U32 in different culture conditions

Sample^a	Raw reads	Mapped reads	Mapping ratio (%)	Genes (RPKM >5)	Expression ratio (%)	Differential genes^b
24B	18,335,500	17,101,379	93.27	6479	69.72	219 up/334 down
24K	17,343,634	16,514,363	95.22	6498	69.92	219 up/334 down
48B	18,740,965	17,564,533	93.72	6324	68.05	1,033 up/847 down
48K	27,441,452	24,162,480	88.05	6483	69.76	1,033 up/847 down

^a24B, 24 h in Bennet medium; 24K, 24 h in Bennet medium with 80 mM nitrate; 48B, 48 h in Bennet medium; 48K, 48 h in Bennet medium with 80 mM nitrate. Mapping ratio: mapped reads/raw reads. Expression ratio: genes (RPKM ≥5)/9,293 genes.
^bGenes expressed under nitrate conditions were compared to those in Bennet medium.

ISSN: 1475-2859