Figure 4
From: Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli
a Optimization of glucose concentration based on the recombinant strain S1 and S5 (S1 and S5 contains only GTs UGT78K1 and ArGt-3, respectively) in 48 h incubation. Maximum conversion of fisetin to respective glycosides was achieved while supplementing 10% additional glucose in the medium. b Production profile of respective glycosides in all the constructed recombinant strains (S1–S9) in optimized concentration of substrate (0.3 mM) and glucose (10%) in 48 h incubation time. Maximum conversion of fisetin to fisetin 3-O-glucoside was achieved to be ~100%. The conversion of fisetin to fisetin 3-O-rhamnoside was ~96%.