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Figure 2 | Microbial Cell Factories

Figure 2

From: Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production

Figure 2

Screening assay to identify heterozygous deletions in yeast which enhance carotenoid biosynthesis. Mat a yeast cells harbouring a chromosomally integrated cassette YEplac195-YB/I/E expressing the GGPP synthase (crtE), the phytoene synthase/lycopene cyclase (crtYB) and the phytoene desaturase (crtI) genes from Xanthophyllomyces dendrorhous were mated with the Mat α yeast deletion strain library. Diploid cells were selected and plated. Colonies exhibiting higher percentage of intensely red colonies were selected out for characterization.

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