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Table 2 Plasmids used in this study

From: Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae

Plasmid

Description

Source or reference

pATP426

Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, URA3 marker, no expression (control plasmid)

Ishii et al., 2014 [29]

pATP426-kivd-ADH6-ILV2

pATP426, co-expression of L. lactis kivd, S. cerevisiae ADH6, and ILV2 genes

Matsuda et al., 2013 [21]

pATP423

Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, HIS3 marker, no expression (control plasmid)

Ishii et al., 2014 [29]

pATP423-MAE1

pATP423, expression of S. cerevisiae MAE1 gene

Matsuda et al., 2013 [21]

pATP423-PMsM

pATP423, co-expression of S. cerevisiae sMAE1, MDH2, and PYC2 genes

Matsuda et al., 2013 [21]