Figure 3

Relative transcriptional levels of mRNA in the recombinant strains. The mRNA expression levels of WT strain is regarded as 1(blank). The relative mRNA expression levels of recombinant strains (filled) are compared with WT strain. (A) Relative transcriptional levels of the pyrR gene. ΔpyrR: the pyrR gene was deleted. (B) Relative transcriptional levels of the prs gene. prs ⁺: the prs gene was overexpressed. (C) Relative transcriptional levels of the pyrG gene. pyrG ⁺: the pyrG gene was constitutively expressed. pyrG ^+*: the pyrG gene was overexpressed. (D) Relative transcriptional levels of the pyrH gene. pyrH ⁺: the pyrH gene was overexpressed. The ccpA gene was used as the internal control gene to normalize the results. Results are the average of three replications with error bars indicating standard error from the mean.