Table 1 Excreted nucleoside concentrations of the strains used in this work
Strain | Genotype | Parental strain | Inosine (mg/L) | Guanosine (mg/L) |
|---|---|---|---|---|
A4 | Wild type | - | 11.1 ± 2.4 | 75.3 ± 4.2 |
A5 | Wild type | - | 6.3 ± 2.3 | 53.3 ± 5.1 |
A6 | Wild type | - | 6.0 ± 4.2 | 64.1 ± 8.2 |
A7 | Wild type | - | 11.4 ± 1.3 | 33.4 ± 2.7 |
A8 | Wild type | - | 5.4 ± 1.0 | 15.3 ± 4.2 |
A9 | Wild type | - | 2.8 ± 1.1 | 81.7 ± 7.1 |
A10 | Wild type | - | 6.6 ± 2.3 | 77.8 ± 4.1 |
I0 | * GPD:ADE4 des | A4 | 12.5 ± 2.0 | 77.2 ± 6.1 |
I1 | GPD:ADE4 des , ΔADE12 | I0 | 144.1 ± 7.3 | 92.8 ± 8.5 |
I2 | GPD:ADE4 des , ΔADE12, ΔIMD3 | I1 | 227.9 ± 9.1 | 70.9 ± 6.2 |
I3 | GPD:ADE4 des , ΔADE12, ΔIMD3, GPD:IMD3 | I2 | 44.51 ± 6.3 | 137.1 ± 3.4 |
I4 | GPD:ADE4 des , ΔADE12, ΔPNP1 | I1 | 268.8 ± 8.1 | 138.0 ± 6.9 |
I5 | GPD:ADE4 des , ΔADE12, GPD:ISN1 | I1 | 145.2 ± 5.1 | 92.2 ± 7.1 |
I6 | GPD:ADE4 des , ΔADE12, ΔIMD3, ΔPNP | I4 | 76.3 ± 4.8 | 27.1 ± 4.3 |
I7 | GPD:ADE4 des , ΔADE12, GPD:GUA1 | I1 | 143.7 ± 6.2 | 91.3 ± 4.5 |
I8 | GPD:ADE4 des , ΔADE12, RIB7p:RIB1 | I1 | 136.4 ± 8.2 | 100.0 ± 4.1 |